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Betametasona crema al 5 -5-5) and in vivo (Rudenko et al., 2014). Our results show that the most prominent effect of 5-HT(2B receptor modulator is the facilitation of formation dendritic spines. This is shown in the results of an analysis with a three-way mixed ANOVA condition (drug) (time), drug dose and dendritic morphology as main effects. Furthermore, the ANOVA showed a significant interaction of the different terms, i.e. 5-HT 2B receptor modulator modifies the level of dendritic spine morphology. The statistical significance is given in the results of a three-way, mixed ANOVA with condition (drug) buy phentermine 37.5 tablets online (time), drug dose and dendritic morphology as main effects. Moreover, the ANOVA shows a significant interaction of the different terms, i.e. 5-HT 2B receptor modulator modifies the level of dendritic spine morphology. In a prior study, the authors showed that level of dendritic spines is altered after a single dose of sertraline (1–2 mg) in the hippocampus (Baldo et al., 2008). We found in the present study that pharmacological enhancement of the 5-HT 2B receptor attenuates formation of dendritic spines after chronic exposure to LSD. This is consistent with our previous results when in vivo microdialysis analysis was used to quantitatively compare changes in dendritic morphology of the caudate and putamen rats injected with LSD versus saline (Cai et al., 2014; Cai 2015). The increase in dendritic spine number that was observed 2 h after drug injection was prevented by pretreatment with the 5-HT 2B receptor inhibitor SB-255055 (see also the results of a multiple comparison t test). Taken together with the pharmacological findings, these data provide evidence of the importance 5-HT 2B receptor in the mechanisms regulating hippocampal synaptic plasticity. Effects of the 5-HT 2B receptor antagonist SB-225858 to block LSD effects were observed in all the rats. However, we did not observe that the effect on LSD effects was attenuated for SSRI, tricyclic antidepressants or MAOI-type drugs (i.e. MAOI, tricyclic antidepressant, and monoamine oxidase inhibitors (MAOIs. These drugs all reduce the level of dopamine D 2 receptor activation but do not influence the serotonergic pathways such as D 3 receptors and serotonin 5-HT 2A receptors. It is plausible that the effects of these drugs might be enhanced in a subgroup of rats (especially the ones with less dopamine D 2 receptor activity) with less serotonin 5-HT 2A receptors and consequently less effects by the serotonin 2B receptor antagonist. 5-HT antagonist SB-225858 (0 mg) significantly reduced the LSD-induced increase in number of dendrites and the spines in CA1 regions of the hippocampus (i.e. prefrontal cortex, posterior cingulate gyrus, anterior gyrus). Thus there were no interactions of the dendritic spine morphology-mediated decrease of LSD-induced activity on the serotonergic pathway with effects of antidepressant. In the brain, dorsal horn is main site of action serotonin. SSRI-type medications decrease the level of serotonin, while MAOIs and tricyclic antidepressant increase serotonin in the cerebrospinal fluid levels (Sugawa et al., 1998). However, the interaction between 5-HT 2B receptor agonist SB-225858 and SSRI-like drugs remains unclear (Tobler et al., 2006; Goto 2013b). In the present study there was a significant interaction of the different terms between interaction (dose, drug and × dose) the number of spines and per dendrite with respect to time: in the acute phase (i.e. 1h), LSD significantly decreased dendritic spine density compared to saline, whereas sertraline significantly increased both the number of dendrites and spine density in the CA1 regions over first 2 h. The latter is consistent with fact that sertraline was a selective serotonin reuptake inhibitor in our test compounds. Thus, the observed reduction of acute and phase LSD/sertraline interaction is consistent with that of a selective serotonin reuptake inhibitor. As sertraline was also a selective MAOI in our test compounds, we did not identify any interaction effects on the serotonergic pathways. There was no interaction between the 5-HT 2B receptor antagonism and the MAOI effects on LSD-induced changes in firing rates the hippocampus. These results are in line with previous vivo microdialysis studies ()
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